The nursery group has agreed a 15-year lease with Sorbon Estates for Cliveden Nursery in Maidenhead.
The deal brings the number of nurseries owned by the chain across Buckinghamshire and Berkshire to six.
Poppies Day Nurseries already owns sites in Bourne End, Old Windsor, Marlow, Beaconsfield and Holmer Green.
The nursery building, located at Cliveden View Shopping Centre, just over a mile from the centre of Maidenhead, has been newly built to incorporate 4,900 square foot of indoor space, as well as a large outdoor play area with sunken sandpit, and a spacious and secure first floor terrace.
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