Glenfield Nursery in Glenburn, Paisley, Nethan Street Nursery and St Roch's Playschool in Glasgow will remain closed this week to contain the spread of the virus.
Two children from Glenfield - a three-year-old and a four-year-old - were found last week to have contracted the virus and two members of staff have shown symptoms. Parents have been given letters with information and guidance on swine flu.
Meanwhile, a four-year-old who attends the morning nursery at Grapenhall Heys Primary School in Warrington, Cheshire, has been diagnosed with the virus after presenting with symptoms last week
The child is believed to have caught swine flu from a close relative who contracted it abroad and who has no association with the school.
The nursery remains open as normal on the advice of the Health Protection Agency. Staff and children who have been in significant contact with the child have been identified and received treatment with Tamiflu, and allowed to continue attending the school and nursery unless they show symptoms.
In a letter sent to parents, Dr Rita Robertson, director of public health at Warrington Primary Care Trust, said, 'Children not identified as being at risk should also continue to attend school as normal.
'Children who attend afternoon nursery are not regarded as being at risk and therefore do not require treatment.'
Davina Woods, headteacher of Grappenhall Heys, said, 'We had a special assembly this morning to teach children about the importance of good handwashing and "safe sneezing", so everyone is well aware of what they need to do to minimise the spread of infection. For now, all pupils have turned up as normal and it really is business as usual.'
The news comes after the World Health Organisation raised the status of the pandemic alert system to Phase 6 last week.
For advice on swine flu, visit:
http://www.direct.gov.uk/en/swineflu/DG_178075
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1241180226578
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Swine flu cases close three nurseries in Scotland
Three nurseries in Scotland have closed temporarily following a diagnosis of swine flu in children attending the settings.